Antibody - Dako mouse monoclonal clone Bu20, cat# M0744 1. Heat tissues to 60°C for 10 mins 2. Deparaffinize and rehydrate tissue sections through series of xylenes and ethanol incubations a. Xylenes or alternative (Citrisolve, Histoclear) incubate 2 times, 5 min each incubation (2 x 5 min) b. 100% ethanol, 5 min c. 95% ethanol, 5 min d. 70% ethanol, 5 min 3. Rinse the slides once in dH20 4. Wash with PBS for 5 mins 5.
GFP Antibody - Clontech mouse monoclonal, clone JL-8 cat# 8371-2
Prepare MOM solutions according to manufacturers instructions- Vector MOM Peroxidase kit (cat# PK-2200) or MOM basic kit (cat# BMK-2202). All incubations take place at room temperature unless indicated.
Heat tissues to 60°C for 10 mins Deparaffinize and rehydrate tissue sections through series of xylenes and ethanol incubations a. Xylenes or alternative (Citrisolve, Histoclear) incubate 2 times, 5 min each incubation (2 x 5 min) b.
GFP Antibody - Molecular probes rabbit polyclonal, cat# A11122 Note: works best on lung, variable on other tissues
Heat tissues to 60°C for 10 mins Deparaffinize and rehydrate tissue sections through series of xylenes and ethanol incubations a. Xylenes or alternative (Citrisolve, Histoclear) incubate 2 times, 5 min each incubation (2 x 5 min) b. 100% ethanol, 5 min c. 5% ethanol, 5 min d. 70% ethanol, 5 min