Protocols

The Krause laboratory has developed a number of protocols to be distributed along with requested reagents. Here, we provide the protocols requested most often. Follow these protocols as a guideline with the realization that depending on the starting materials, optimization may be needed.

Available protocols

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All Y-Chromosome FISH Immunohistochemistry

BRD-U Immunohistochemistry on Mouse - Paraffin

Basic FISH Protocol - Paraffin

CD45 GFP CK immunofluorescence on mouse - paraffin

GFP Immunohistochemistry on Mouse - Paraffin

Preparing Y-Chromosome Probe

Preparing slides for FISH

Protocol for preparation of slides with bone marrow or peripheral blood for fluorescence in situ hybridization

Basic FISH Protocol - Frozen